Blasticidin S

Molecular Formula

C17H26N8O5 (HCl)

M.W.

458.90

CAS number

3513-03-9

Details

Source

Streptomyces griseochromogenes

Fermentek product Code

BLS-001

Brand/grade

For research

Specifications

Appearance

Off-white

Purity by HPLC

≥98% ; refer to CoA for more data

Purity By TLC

Single spot on TLC plates, by multiple methods.

Melting point

Gradual decomposition with charring between 210°C to 250°C ; (For your information only, not spec)

Crystallinity

Amorphous

Solubility test

Clear colorless solution at 10 mg/ml Acetic acid or Water.

Chemical identification

Names and identifiers

Synonyms:

Bla-S
Blasticidin S, monohydrochloride (8CI)

IUPAC Name:

(2S,3S, 6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylicacid; hydrochloride

RTECS: EC4900000

EU number

609-067-6

Description

Blasticidin S Hydrochloride belongs to a group of cytosine amino nucleoside antibiotics.

The compound Blasticidin S has a wide spectrum of antimicrobial activity and acts on prokaryotic and eukaryotic organisms by inhibiting protein synthesis.

InChl Key

YQXYQOXRCNEATG-ZAYJLJTISA-N

Canonical SMILES

N(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

Isomeric SMILES

CN(CC[C@H](CC(=O)N[C@H]1C=C[C@@H](O[C@@H]1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

Further Information

Solubility ( literature )

Soluble in water, Acetic acid. Slightly soluble in acidified methanol.

Compound Classification

Cytosine amino nucleoside type antibiotic

Storage, handling

Store in a freezer upon arrival, at -10°C to -25°C

Retest time

3 Years

Applications

Blasticidin-S is a commonly used tool in genetic engineering. Blasticidin-S inhibits protein synthesis in both prokaryotic and eukaryotic cells. It is common method to include a gene for Blasticidin S resistance into the plasmid being inserted into the target cells, and then to apply Blasticidin S to the culture to select these cells that have acquired that immunity. The Blasticidin S gene resistance gene (BSR), found in Bacillus cereus K55-S1, has been used in multiple studies for its use as a selective compound. Another antibiotic that belongs to the family of nucleosides is Puromycin.

Disclaimer

Refer to MSDS for further safety and handling instructions.

Composition

Ingredient type

Fermentek product

Special Info

Available since

2015