Blasticidin S
Molecular Formula
C17H26N8O5 (HCl)
M.W.
458.90
CAS number
3513-03-9
Source
Streptomyces griseochromogenes
Fermentek product Code
BLS-001
Brand/grade
For research
Appearance
Off-white
Purity by HPLC
≥98% ; refer to CoA for more data
Purity By TLC
Single spot on TLC plates, by multiple methods.
Melting point
Gradual decomposition with charring between 210°C to 250°C ; (For your information only, not spec)
Crystallinity
Amorphous
Solubility test
Clear colorless solution at 10 mg/ml Acetic acid or Water.
Names and identifiers

Synonyms:

  • Bla-S
  • Blasticidin S, monohydrochloride (8CI)

IUPAC Name:

(2S,3S, 6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylicacid; hydrochloride

RTECS: EC4900000

EU number
609-067-6
Description

Blasticidin S Hydrochloride belongs to a group of cytosine amino nucleoside antibiotics.

The compound Blasticidin S has a wide spectrum of antimicrobial activity and acts on prokaryotic and eukaryotic organisms by inhibiting protein synthesis.

InChl Key
YQXYQOXRCNEATG-ZAYJLJTISA-N
Canonical SMILES
N(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
Isomeric SMILES
CN(CC[C@H](CC(=O)N[C@H]1C=C[C@@H](O[C@@H]1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
Solubility ( literature )

Soluble in water, Acetic acid. Slightly soluble in acidified methanol.

Compound Classification

Cytosine amino nucleoside type antibiotic

Storage, handling
Store in a freezer upon arrival, at -10°C to -25°C
Retest time
3 Years
Applications

Blasticidin-S is a commonly used tool in genetic engineering. Blasticidin-S inhibits protein synthesis in both prokaryotic and eukaryotic cells. It is common method to include a gene for Blasticidin S resistance into the plasmid being inserted into the target cells, and then to apply Blasticidin S to the culture to select these cells that have acquired that immunity. The Blasticidin S gene resistance gene (BSR), found in Bacillus cereus K55-S1, has been used in multiple studies for its use as a selective compound. Another antibiotic that belongs to the family of nucleosides is Puromycin.

Disclaimer
Refer to MSDS for further safety and handling instructions.
Ingredient type
Fermentek product
Available since
Title
Transport information
Value
Pg II
Title
REACH status
Value
On REACH List of Pre-Registered Substances, March 2009 Registration Date: 31-MAY-2018
Title
Deleted CAS numbers
Value
885319-52-8
Fermentek Product Category
Signal to sort
B