DNA replication inhibitors

7-Amino-Actinomycin D (7-AAD)

Molecular Formula

C62H87N13O16

M.W.

1 270.43

CAS number

7240-37-1

MSDS

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Details

Source

Semisynthetic Derivate of Actinomycin D

Fermentek product Code

7AD-001

Brand/grade

For research

Specifications

Appearance

Purple powder

Purity by HPLC

≥97% ; refer to CoA for more data

Purity By TLC

≥98% ; refer to CoA for more data

Melting point

235°C-255°C

Solubility test

Clear purple solution at 20mg/ml DMSO

Chemical identification

Names and identifiers

IUPAC name:
2,7-Diamino-N,N'-bis[(6S,9R,10S,13R,18aS)-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1,4,7,10,13]oxatetraazacyclohexadecin-10-yl]-4,6-dimethyl-3-oxo-3H-phenox azine-1,9-dicarboxamide

RTECS:

AU1579000

EU number

635-285-6

Chemical name

7-Amino-Actinomycin-D

Description

(7-AAD) 7-Amino-Actinomycin-D is produced synthetically from natural Actinomycin D. (7-AAD) 7-Amino-Actinomycin-D binds to single stranded DNA. it is used, as a fluorescent dye, as a reagent capable to distinguish dead and live cells.

InChl Key

YXHLJMWYDTXDHS-UHFFFAOYSA-N

Canonical SMILES

CC1C(C(=O)NC(C(=O)N2CCCC2C(=O)N(CC(=O)N(C(C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=CC(=C(C4=C3N=C5C(=C(C(=O)C(=C5O4)C)N)C(=O)NC6C(OC(=O)C(N(C(=O)CN(C(=O)C7CCCN7C(=O)C(NC6=O)C(C)C)C)C)C(C)C)C)C)N

Further Information

Solubility ( literature )

Dichloromethane, DMSO, Methanol. Slightly soluble in water

Storage, handling

Store in a freezer upon arrival, at -10°C to -25°C

Hygroscopic substance.

Protect from moisture

Use the original container to store the product.

Keep the lid tightly closed.

Avoid exposing to strong direct light.

Retest time

3 Years